Nope, was never used up - from 1st version on - BUT! the high stats you gain now are new.
Well I made a heck of a lot of potions to see if there was a limit. Since I was carrying about 30 each of the plants I could make lots! Each Stat gained about 15 per potion and Health/Mana gained about 800 per potion. I would have expected the holy water to vanish after one use.
