Best plot twist: KotOR
Worst plot twist: NWN OC
Best fight: nothing stands out for me.
Worst fight: Emerald weapon in FFVII. Talk about stupid and tedious.
Best city: Athkatla in BG2.
Worst city: any city in Diablo 2.
Best NPC: Uhm. A tie between Morte (PST), Dak'kon (PST), Jolee Bindo (KotOR) and Canderous Ordo (KotOR). For non-joinable, Ravel from PST and Yuthura Ban from KotOR.
Worst NPC: Minsc, for sheer overrated factor. T3-M4 for a lack of personality. Cernd for blandness. Malak for the manic laughter and cliched, cartoonish villainy.
Worst quest or sidequest: most of the quests in the first village in ToEE.
The rest I'm not terribly interested in answering, though. Eh.