[Piracy]
[color:"orange"]There's simply no way of knowing for sure.[/color]
IIRC during BD development, in a discussion about copy protection, one of the Larians mentioned that a particular game (without copy protection) was significantly outsold by its expansion pack (which did use copy protection). At least in this case, the copy protection companies have an example where a known minimum number of people had to have pirated the original game, and presumably if they were willing and able to buy the expansion, they would have been able to buy the game.
In general, estimates of losses due to piracy may be more self-serving than realistic, depending on the source.