I lost mine as well at the end of Act 1. They probably added another new location that completely remove all your buff again and if you eat it before getting to that part, byebyebloodrose.

Two things I did before it was gone was

1) Final Fight. Got the 50 turn encoraged buff. (TBh, this kind of thing is never good because cleansing will happen)

and

2) freed Slane

I doubt it has to do with Slane so I have a feeling it has to do with the final act.

Man, this bug is as bad as Fane losing his mask on Act 2 if you don't take it off him first and put it in your inventory because he will replace it with a hood.