If the same actions in the same locations have different performance in the playthroughs (and it isn't a case of differing mods), most likely the problem is in the save itself.
Please email supportdos2@larian.com with your latest save (or a Dropbox or Google drive link, etc), as well as the modsettings.lsx file from your profile folder.
Each save is a folder in the '..\Documents\Larian Studios\Divinity Original Sin 2 Definitive Edition\PlayerProfiles\<ProfileName>\Savegames\Story' folder.
In Windows Explorer you can zip a file or folder by right clicking on it/them and selecting 'Send To | Compressed (zipped) Folder'.
